Fig. 4

Effect of miR-214-3p on cellular proliferation, migration and invasion. (A) Cell proliferation (MTT) assay following transfection of pre-miR-214-3p in a)TE7, b) FLO-1, and c) SK-GT-4 cells. B. Cells (TE7,FLO-1 and SK-GT4) were transfected for 48 hours with pre-miR-214-3p (50 nM), followed by performance of (a) migration and (b) invasion assays in real time using XCELLigence-RTCA system. Changes in (C-a) migration and (C-b) invasion in hESO cells after silencing miR-214-3p. Cells were transfected with anti-miR-214-3p (25 nM) for 48 h prior to performance of the assay. Error bars represents ± S.D. and statistical significance is indicated by * (p < 0.05). Representative bar graphs for three independent experiments are shown