Fig. 7

CX3CL1 inhibits ccRCC cell proliferation, migration, and invasion by inducing ferroptosis in vitro. A 786-O cells were incubated with Fer-1 (0, 1, 2, 4 μM/ml) for 48, 72, 96, and 120 h; then, cell viability was estimated by CCK-8 assay, and cell proliferation of 786-O cells in normal control, OE-CX3CL1, and OE-CX3CL1 + Fer-1 groups was estimated by CCK-8 assay. B Cell colonies of 786-O cells in normal control, OE-CX3CL1, and OE-CX3CL1 + Fer-1 groups. C-D 786-O cell migration and invasion assays were performed in normal control, OE-CX3CL1, and OE-CX3CL1 + Fer-1 groups. E Western blotting of CX3CL1, PCNA, GPX4, and XCT proteins. F The immunostaining assay identified the subcellular localizations of CX3CL1, PCNA, GPX4, and XCT. Scale bar: 10 μm. *P < 0.05, **P < 0.01, ***P < 0.001