Fig. 6
From: Methionine regulates self-renewal, pluripotency, and cell death of GIC through cholesterol—rRNA axis
A, C Volcano plot showing the miRNA distribution in control versus methionine-depleted groups (A) and in control versus statin-treated groups (C). Up- (red color-positive fold change) and down-regulation (green color-negative fold change) in the treated group compared to the control group are indicated. Hierarchical clustering of the differentially expressed miRNAs (P value ˂ 0.05 and change ≥ 2.0 fold or ≤ − 2.0 fold) in the treatment group compared with the control group showed a clear distinction between control and treatment groups in miRNA including small noncoding RNAs. B, D Treatments are methionine depletion (B) and simvastatin administration for 48 h (D). Color scale indicates the relative expression of miRNAs: red shows higher expression and blue lower expression. E GSEA of differentially expressed genes. The enrichment profiles for “gene ontology cytosolic large ribosomal subunit” of three cell lines are shown. Heat map of top 20 genes from GSEA are shown on the right. Red column corresponds to upregulated genes and blue column corresponds to downregulated genes. NES: normalized enrichment score, FDR-q: false discovery ratio. F Methionine depletion reduced protein synthesis. We can detect the 5 FAM-Azide labeled protein in only cultured with methionine contained medium