Fig. 2

H2A.Z silencing enhances SFRP1 expression in ICC cells. A, C The relative levels of H2A.Z and SFRP1 expression were determined in ICC and nontumor HIBEpic cells by quantitative RT–PCR and Western blotting. B H2A.Z silencing and related changes in SFRP1 expression were demonstrated in HuccT-1, RBE and HIBEpic cells by quantitative RT–PCR and Western blotting. D SFRP1 overexpression and H2A.Z expression were demonstrated in HuccT-1, RBE and HIBEpic cells by quantitative RT–PCR and Western blotting. The data shown are representative images, individual mean values or the mean ± SD of each group in three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001