Fig. 1

Upregulation of H2A.Z is associated with downregulation of SFRP1 and poor survival. A-B The levels of H2A.Z and SFRP1 in 25 primary ICC and paired adjacent nontumor tissues were examined by IHC. Scale bar, 100 μm. B The expression levels of H2A.Z (upper panel) and SFRP1 (lower panel) were semiquantitatively analysed. C The mRNA transcript levels of H2A.Z and SFRP1 were measured by quantitative RT–PCR in 20 pairs of ICC and paracancerous tissues, and Pearson correlation coefficients were calculated (r =  − 0.6776, **p = 0.001). D The mRNA transcript levels of H2A.Z and SFRP1 were quoted from the GSE76297, and Pearson correlation coefficients were calculated (r =  − 0.04623, ****p = 0.001). E Kaplan–Meier analysis of overall survival in 50 ICC patients with higher H2A.Z (n = 25) and lower SFRP1 (n = 25) expression vs. patients with lower H2A.Z (n = 25) and higher SFRP1 (n = 25) expression. The data shown are representative images, individual mean values or the mean ± SD of each group in three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001