Table 2 Antibodies used for Western blotting
Antibody | Company | Catalog number | WB |
|---|---|---|---|
AKT rabbit mAb | Cell signaling | 4691 | 1:1000 |
p-AKT (Ser473) rabbit pAb | Cell signaling | 9271 | 1:1000 |
mTOR rabbit mAb | Cell signaling | 2983 | 1:1000 |
p-mTOR (Ser2448) rabbit mAb | Cell signaling | 5536 | 1:1000 |
ERK1/2 rabbit pAb | Cell signaling | 9102 | 1:1000 |
p-ERK1/2 (Thr202/Tyr204) rabbit mAb | Cell signaling | 4370 | 1:2000 |
α- tubulin mouse mAb (HRP) | Abcam | ab40742 | 1:10,000 |
gt-anti-rb IgG peroxidase | Sigma | A6154 | 1:2000 |