Fig. 2

Proliferation studies and Western-blot analysis of ECC cell lines. A Effects of AKT inhibitor MK-2206 on EGI1 (a), CCC5 (b), and TFK1 (c) after 0, 24, 48, 72 and 96 h (h). 0 h values were set to 100%. Concentrations of MK-2206 are indicated. Shown are the mean values of n = 3–5 independent experiments with each 6–8 replicates. Controls were treated with solvent (DMSO). Significance was tested by one-way ANOVA between the control and treatment groups. *p < 0.05, **p < 0.01, ***p < 0.001 with 95% level of confidence. B Cropped Western blot images of 25 µM MK-2206-treated EGI1 and CCC5 with specific antibodies against total AKT and p-AKT, mTOR and p-mTOR, ERK1/2 and p-ERK1/2 after 3, 8 and 24 h. α-Tubulin was used as loading control. Note reduced phosphorylation of AKT, and increase in p-ERK1/2