Fig. 2

Macrophage cells displayed the M2 phenotype when the co-culture was achieved by using LSCC cells. Macrophage was co-cultured with TU212 cells or TU177 cells for 48 h. A Inverted microscopy images the morphology of macrophage. B Expression of CD163 and CD206 mRNA was detected by RT-PCR. C Characterization of macrophage phenotype by cell surface markers. Macrophage cells were stained in terms of CD68. Cells were gated in terms of CD68 representative survey results were presented. D and E The expression of lncRNA HOTAIR in macrophages was examined by RT-PCR and Western Blotting. F CCL18 and IL-10 levels in macrophage supernatants under the ELISA-based measurement. Data are expressed as mean ± SEM, n = 3 independent experiments. (*p < 0.05, *** p < 0.001 were considered statistically significant). Original gels are presented in Supplementary Fig. 2-E-HOTAIR and Fig. 2-E-GAPDH