Fig. 4

C9orf16 knockdown inhibited cancer cell invasion and increased apoptosis. A Expression of C9orf16 in pancreatic cancer cell lines and normal epithelial cells was examined by RT-PCR. B RT-PCR examination of C9orf16 knockdown cells and the scrambled cells showed the C9orf16 knockdown efficiency. C Real time PCR was used to check the C9orf16 knockdown efficiency. D-E Western blotting was performed to confirm the C9orf16 knockdown efficiency. F Cell proliferation measured by MTT assay showed decreased cell proliferation in PANC-1 cells with oeC9orf16 knockdown. Migration and invasion assays (G) and cell number quantification (H) of the C9orf16 knockdown and the scrambled cells. Flow cytometry analysis (I) of Cisplatin induced cell apoptosis and quantification of late apoptotic cell percentage (J) of the C9orf16 knockdown and the scrambled cells. Scale bar: 100 µm. **: p < 0.01; ***: p < 0.001; ****: p < 0.0001