Fig. 6

CircBRWD3 promotes tumorigenesis of breast cancer via miR-142/RAC1-PAK1 signaling. A Nude mice were subcutaneously injected with the breast cancer cells transfected with indicated constructs (sh-NC; sh-circBRWD3; OE-vector; OE-circBRWD3). After 35 days, the animals were sacrificed and the tumor volume was measured. ^**P < 0.01, Sh-NC vs. Sh-circ group; mock vs. circBRWD3 group. B Nude mice were subcutaneously injected with the breast cancer cell transfected with indicated constructs (sh-NC; sh-circBRWD3; OE-vector; OE-circBRWD3). After 35 days, the animals were sacrificed and the tumors was weighted. ^**P < 0.01, Sh-NC vs. Sh-circ group; mock vs. circBRWD3 group. C The protein levels of RAC1, PAK1, and p-PAK1 in (B) were determined using western blot. D The protein levels of E-cadherin, N-cadherin, Vimentin, and MMP9 in (B) were determined using western blot. E Schematic representation of the molecular mechanism of EIF4A3/circBRWD3/ miR-142-3p_miR-142-5p /RAC1/PAK1 axis in breast cancer. All experiments were carried out in triplicate. Sh-circ: sh-circBRWD3; E-cad: E-cadherin; N-cad: N-cadherin