Fig. 2

CircBRWD3 promotes breast cancer tumorigenesis in vitro. A The mRNA expression level of circBRWD3 in breast cancer cell lines (MDA-MB-231 as well as BT-549) transfected with OE-vector or OE-circBRWD3 plasmids and sh-NC or sh-circBRWD3 was calculated by qRT‐PCR. ^**P < 0.01, Sh-NC vs. Sh-circ group; mock vs. circBRWD3 group. B The cell proliferation in (A) was determined using CCK-8 assay. ^**P < 0.01, Sh-NC vs. Sh-circ group; mock vs. circBRWD3 group. C The cell viability in (A) was determined through EdU assay. ^**P < 0.01, Sh-NC vs. Sh-circ group; mock vs. circBRWD3 group. D The colony number of cells in (A) was calculated using colony formation assay. ^**P < 0.01, Sh-NC vs. Sh-circ group; mock vs. circBRWD3 group. E The cell migration capability in (A) was determined using transwell assay. ^**P < 0.01, Sh-NC vs. Sh-circ group; mock vs. circBRWD3 group. F. The apoptosis of cells in (A) were assessed by flow cytometry analysis.^**P < 0.01, Sh-NC vs. Sh-circ group; mock vs. circBRWD3 group. All experiments were carried out in triplicate. Sh-circ: sh-circBRWD3