Fig. 1

CircBRWD3 is upregulated in breast cancer tissues as well as cell lines. A The volcano plot of differentially expressed circRNAs in 5 pairs of normal mammary gland tissues and breast cancer tissues specimens based on the GEO database. B Clustered heatmap showing differentially expressed circRNAs in 5 pairs of normal mammary gland tissues and breast cancer tissues specimens in GEO database. C Schematic illustration of circBRWD3 formation by back splicing from the BRWD3 gene. D The expression level of circBRWD3 in 66 pairs of breast cancer tissues and adjacent noncancerous specimens was calculated using qRT‐PCR. ^**P < 0.01, Tumor vs. Normal. E Kaplan–Meier survival analyses of breast cancer patients with low or high circBRWD3 expression. F The expression level of circBRWD3 in four breast cancer cell lines (BT-549, MDA-MB-231, SUM-159, and MDA-MB-453) and the human normal breast epithelial cell line (MCF-10A) was quantified by qRT‐PCR. ^**P < 0.01, MCF-10A vs. other cell lines. G The expression level of circBRWD3 and BRWD3 in breast cancer cell lines (MDA-MB-231 and BT-549) with or without RNase R treatment was calculated through qRT‐PCR. ^**P < 0.01, mock vs. Rnase R in circBRWD3 group. H The existence of circBRWD3 was confirmed in breast cancer cell lines (MDA-MB-231 and BT-549). All experiments were done in triplicate