Fig. 4

FAM99A suppresses the proliferation ability of HCC cells in vitro. A Relative expression level of FAM99A in five HCC cell lines. B The overexpression efficiency of Lv-FAM99A was examined using qRT-PCR in Hep 3B and Huh-7 cells. C The knockdown efficiency of sh-FAM99A was examined using qRT-PCR in Hep 3B and Huh-7 cells. D CCK-8 assay showed that upregulation of FAM99A inhibited the proliferation of Hep 3B and Huh-7 cells. E CCK-8 assay indicated that downregulated FAM99A promoted the proliferation of Hep 3B and Huh-7 cells. F Colony formation assays showed that overexpressing FAM99A reduced the number of colonies. G Colony formation assays revealed that knocking down of FAM99A increased the number of colonies. All experiments were repeated three times. *P < 0.05; **P < 0.01; ***P < 0.001