Fig. 5

Western blot analysis of proteins in whole cell extracts obtained from cells cultured in the presence of K164 (concentrations of 10 μM, 20 μM, and 50 μM) after 48 h of incubation. Preparation of cell extracts and protein detection are described in Materials and Methods. Densitometry analysis was used to quantify all bands, and their intensity was normalized with respect to β-actin. For cell lines cultured in the absence of K164, the ratio of the examined proteins to β-actin was assumed to be 1. Panel A: PARP, AKT1, p 53, and B-cell lymphoma 2 (BCL-2) family proteins (pro- and anti- apoptotic). Panel B: phosphorylated proteins