Fig. 1

Fraction of multiple mutations in individual genes. a Percentages of HCCs with single mutation (SM) and multiple mutations (MMs) according to the classification for oncogenes and tumor suppressor genes (TSGs). b Comparison of the impact of genomic variant annotations and functional effect by SnpEff. c A comparison of TMB between MMs tumors and SM tumors. The TMB was significantly higher in MMs tumors than that in SM tumors. d Comparisons of signature contributions between MMs tumors and SM tumors. Three signature scores were significantly varied betweem MMs tumors and SM tumors. e Prognostic analysis according to the presence of MMs. The RFS was significantly worse in the group with MMs tumors than in the group with SM tumors (P = 0.012, log-rank test)