Fig. 9

Experimental validation of SNHG1 and SNHG7 in HCC cell lines. A Univariate Cox analysis revealed that SNHG1 and SNHG7 were independent prognostic factors for HCC. B qRT-PCR analysis showed that the expression of SNHG1 was increased in the hepG2 and Huh7 cell lines, and the expression of SNHG7 was upregulated in most HCC cell lines. C Smart silencer-mediated knockdown of SNHG1 and the siRNA-mediated knockdown of SNHG7 were confirmed using qRT-PCR. D The results of the FA metabolism PCR array show that the expression of 5 genes (ECHS1, MCEE, ACOT12, CPT1B, and BDH2) were up-regulated and that of 17 genes ACSBG1, FABP6, ACADVL, ACSM3, ACOX2, CPT2, ECI2, ECHS1, DECR, SLC27A6, MUT, SLC27A4, ACAD10, FASN, ACSL4, ACADSB, and GK2 were down-regulated after the knockdown of SNHG1 in the HepG2 (I Fold change I > 2). E The results of the FA metabolism PCR array show that the expression of GK was up-regulated, while that of 14 genes (ACAA1, SLC27A4, ACSBG1, PRKAG3, FABP5, ACSL1, CPT1C, MUT, ACAD11, GPD2, ECHS1, GK2, CPT2, and CPT1B) were down-regulated after silencing SNHG7(I Fold change I > 2). F GSEA analysis revealed that the signature was negatively regulate the fatty acid beta-oxidation, and fatty acid beta-oxidation using CoA oxidase. G ACSBG1 expression was increased after the knockdown of SNHG1 in the HepG2 and Huh7 cell lines, and ACSBG1 expression was increased after the knockdown of SNHG7 in the HepG2 and Huh7 cell lines. H The cells were stained with Oil Red O kit and photographed to lipid droplets. I GPX4, NRF2, NCOA4, and CD98 expressions were downregulated and that of KEAP1 was upregulated after the knockdown of SNHG1 in the HepG2 and Huh7 cell lines. J GPX4, NRF2, NCOA4, and CD98 expressions were downregulated and that of KEAP1 was upregulated after the knockdown of SNHG1 in the HepG2 and Huh7 cell lines. *, P < 0.05, ****, P < 0.0001. The original blots/gels are presented in Supplementary Fig. 2