Fig. 6

SMI#9 induces monopolar mitotic spindles. MDA-MB-468 (A, B) and HCC1937 (C, D) TNBC cells were treated with 2.5 or 10 nM PTX, respectively, 0.75 µM SMI#9 or SMI#9 + PTX and analyzed for TAU (green) and α-tubulin (red) expression/localization by immunofluorescence staining. Bar, 10 µm. Enlarged images of α-tubulin stained mitotic spindles are shown. B, D Quantitation of total TAU positive cells and those with monopolar mitotic spindles. Results are expressed as mean ± S.E.M of replicates. B *P < 0.05, C *P < 0.01