Fig. 2

Effects of miR-200a-3p on function of YAP are differentially related to HPV statuses. Intersection Venn diagram for potential downstream targets of miR-200a-3p delivered from 5 bioinformatics Databases (up), and schematic depiction of the double-strand formation of miR-200a-3p with the 3’-UTR of YAP (below) (A). Negative correlation between miR-200a-3p and YAP expression was analyzed through the starBase 3.0 (B). Schematic diagrams of human YAP 3’-UTR luciferase constructs with YAP-3’UTR and miR-200a-3p target sequences (C). Relative luciferase activities in C33A (D), Siha (E) and Hela (F) with co-transfection of YAP and miR-200a-3p, respectively. Significant decreases in levels of YAP mRNA (G) and YAP protein (H) in C33A and Siha cells following up-regulation of miR-200a-3p, respectively. Significant increases in levels of YAP mRNA (I) and YAP protein (J) in C33A and Siha cells following knockdown of miR-200a-3p, respectively. *p < 0.05, **p < 0.01, ***p < 0.001, ns: not significant.