Fig. 5

MiR-575 promoted ESCC cells proliferation, migration and invasion in vitro by targeting ACAA2. A Relative mRNA expression and protein level of ACAA2 were evaluated by RT-qPCR and western blot analysis in EC109 cells transfected with the miR-575 mimics. B CCK-8 assays were performed to determine the ability of proliferation in EC109 cells transfected with miR-575 mimics, miR-NC, pc-ACAA2, pc-NC, pcACAA2 + miR-NC and pcACAA2 + miR-575 mimics. C The cell migratory and invasive capabilities were assessed by transwell assays in EC109 cells transfected with miR-575 mimics, miR-NC, pc-ACAA2, pc-NC, pcACAA2 + miR-NC and pcACAA2 + miR-575 mimics. Scale bar, 50 μm. Data were showed as mean ± SD. *P < 0.05, **P < 0.01