Fig. 2
From: Clinical significance and oncogenic function of NR1H4 in clear cell renal cell carcinoma
Knockdown of NR1H4 suppressed proliferation, migration and invasion of ccRCC cells. A Immunohistochemistry assay showed the higher expression of NR1H4 in ccRCC tumor tissues when compared with tumor adjacent tissues. B-E Analysis of NR1H4 expression in 3 paired ccRCC specimens and ccRCC cell lines through quantitative real‐time PCR and western blot. F qRT-PCR and western blot verified the knockdown of NR1H4 in 786-O cells via siRNA. NR1H4 downregulation inhibited proliferation of 786-O cells, as demonstrated by (G) CCK-8 assay, (H) clone formation assay and (I) EdU assay. NR1H4 knockdown attenuated the migration and invasion ability of ccRCC cells indicated by transwell assay (J) and wound healing assay (K). OD, optical density; EdU (5-ethynyl-2' -deoxyuridine); *p < 0.05; **p < 0.01; ***p < 0.001