Fig. 2
TNBC cells induced platelet aggregation. Representative traces from light transmission aggregometry showing platelet aggregation induced by TNBC cells at different numbers (A). Statistical analysis of the effect of TNBC cells on platelet function (B, C). Data generated with Hs578T and Hs578Ts(i)8 cells was co-analysed together to determine if there were significant differences between the two cell line variants (C). Percentage of platelets available that aggregation is graphed. Graphs represent mean ± SEM from n = 4 independent experiments. One-way ANOVA was performed for (B) and (C) and a two-way ANOVA performed for (D). ****p < 0.0001 vs control (platelets stirred in the absence of cancer cells) and ##p < 0.01 (i.e., this significant result is from the ANOVA tests that were performed, with an ## used instead of ** to highlight the significant difference between 1000 and 250 cells. Representative micrographs from optical microscopy demonstrating the presence of big aggregates in the presence of both cell lines and the absence of aggregates in stirring platelets. Scale bar 200 μm (E). For complete two-way ANOVA results see Supplemental Material