Table 1 Primer sequence and synthesis condition
GENE | Primer | Length (bp) | Tm (degrees centrigade) |
|---|---|---|---|
GAPDH | ACAACTTTGGTATCGTGGAAGG | 101 | 60.7 |
GCCATCACGCCACAGTTTC | 60.54 | ||
BUB1 | GGAGAAGGAGCCTTTGCCCAG | 117 | 60.14 |
GTAGAATTCCCAGGGGTTGGCAG | 60.62 | ||
BUBR1 | CTTCTCCCTACCAGGTAGACCTG | 173 | 60.77 |
GGCATTCAGAATCCGCACAAAG | 60.75 | ||
MAD1 | GAGTCTGCCATCGTCCAAGGAG | 157 | 60.03 |
CGTGGTGATGTCGATCTGGTAGC | 60.04 | ||
CDC20 | CTCAGGCCATGGCTTTGCACAG | 158 | 60.99 |
TCAGGGTCTCATCTGCTGCTGC | 61.65 | ||
TTK | GGGACCCAAAACAGAGGATATCC | 134 | 60.8 |
ACCAACAAGTTGGCCCAGAAC | 60.82 | ||
LIS1 | TCTTGCTGTCTGGATCCAGAGAC | 156 | 60.8 |
GGGTCTTGTCATCAGCACAACTC | 60.82 | ||
DYNC1H1 | TGGAAGTCAACGTCACCACCTC | 128 | 60.8 |
GGTTGAGATGGCATTGGACAGTG | 60.82 | ||
DYNLL1 | CTCAGGCGCTGGAGAAATACAAC | 95 | 60.8 |
GATGCAATGCCAGGTGGGATTG | 60.82 |