Table 1 The primer sequence used in this study
From: Lnc-SELPLG-2:1 enhanced osteosarcoma oncogenesis via hsa-miR-10a-5p and the BTRC cascade
Primer name | Sequence | Product length |
|---|---|---|
lnc-SELPLG-2:1-F | 5′ CAAGGAACTGTGCTGGGATT 3’ | 246 bp |
lnc-SELPLG-2:1-R | 5′ AGCAGTAGCGGGATGAGAAA 3’ | |
GAPDH-F | 5′ GAGTCAACGGATTTGGTCGT 3’ | 185 bp |
GAPDH-R | 5′ GACAAGCTTCCCGTTCTCAG 3’ | |
lnc-DDX47–3:1-F | 5′ AGGTGGCAGCTTCAGAAAAA 3’ | 195 bp |
lnc-DDX47–3:1-R | 5′ AGCATCCTTGGAACATCCTG 3’ | |
lnc-SRSF2–9:2-F | 5′ CCTGGTCGACACCTTGATTT 3’ | 179 bp |
lnc-SRSF2–9:2-R | 5′ AAAGTCCCAGGGTCAGTGTG 3’ | |
lnc-SAMD11–1:1-F | 5′ CCAGAAGGTCAGCCGTAGAG 3’ | 190 bp |
lnc-SAMD11–1:1-R | 5′ CCACACCAGGAAACCTGTCT 3’ | |
lnc-PPP1R9B-4:2-F | 5′ TGGCCCTCCTGTAAGTATGC 3’ | 182 bp |
lnc-PPP1R9B-4:2-R | 5′ TGTGTACCCCTCACCCTCTC 3’ | |
lnc-ZNF77–165:3-F | 5′ GGACTTTCGGAAAAGCCTTC 3’ | 226 bp |
lnc-ZNF77–165:3-R | 5′ CCCCAGTAGCTGGGACTACA 3’ | |
INPPL1-F | 5′ CTGGGTGACCTGACCAAGAT 3’ | 224 bp |
INPPL1-R | 5′ ATGAAGTCCTTGCGCTGAGT 3’ | |
HOXA1-F | 5′ GGGTGTCCTACTCCCACTCA 3’ | 162 bp |
HOXA1-R | 5′ GGACCATGGGAGATGAGAGA 3’ | |
MAP3K7-F | 5′ ACAGTGTTCCCAAGGAGTGG 3’ | 196 bp |
MAP3K7-R | 5′ AACTTCAGGTGCCATCCAAG 3’ | |
BTRC-F | 5′ TGTCATACCTGGATGCCAAA 3’ | 163 bp |
BTRC-R | 5′ TACTGTCCCCATCCTCTTCG 3’ | |
U6-F | 5′ CGCTTCGGCAGCACATATAC 3’ | 83 bp |
U6-R | 5′ CGAATTTGCGTGTCATCCTTG 3’ |