Fig. 5

Deficiency of Notch signaling leads to increased chemokine expression in CRC tumor microenvironment. A RT-PCR result showing decreased NOTCH1 expression in MC38 cell line stably transected with shNOTCH1 (***p < 0.001). B WB result showing decreased NOTCH1 protein level in MC38 cell line stably transected with shNOTCH1. Full-length blot is presented in the supplementary material. C Gene sets enriched in the control and knock-down groups. D The expressions of Cx3cl1, Cxcl1 and Ccl9 in the control and knock-down groups (**p < 0.01, ***p < 0.001). E The expression levels of CX3CL1 and CXCL1 in the WT and mutation patients of TCGA (ns, not significant, ***p < 0.001). F The infiltration scores of different immune cells in the WT and mutation patients of CRC in TCGA (*p < 0.05, ***p < 0.001)