Fig. 5

Overexpression of SRPK1 attenuated inhibition of miR-659-3p in osteosarcoma cells growth, migration, and invasion. A Real-time PCR showed that overexpression of SRPK1 restored SRPK1 mRNA expression in miR-659-3p mimic transfected HOS cells. B Western blot confirmed that overexpression of SRPK1 restored SRPK1 protein level in HOS osteosarcoma cells (lane 4). SRPK1 expression was knocked down by miR-659-3p mimic (lane 2). SRPK1 and GAPDH western blots were performed on different gels. C Overexpression of SRPK1 increased HOS cells growth (Green line and Blue line, P < 0.001 n = 3), and attenuated miR-659-3p inhibition of HOS cells growth (Purple line and Red line, P < 0.001 n = 3). D Quantification of HOS cells wound gap after 12 and 24 h culture. E Representative pictures of HOS cells wound gap after 12 and 24 h culture showed that overexpression of SRPK1 accelerated wound healing in the dishes. F Transwell invasion assay showed that inhibition of SRPK1 expression by miR-659-3p mimic decreased HOS cells invasion and overexpression of SRPK1 increased HOS cells invasion. G Quantification of cell numbers in the lower chambers of transwells. H Representative FACS histogram plots showed that SRPK1 overexpression decreased G1/G0 phase arrest induced by miR-659-3p. I The average percentage of each phase in cell cycle (n = 3 each group) showed that SRPK1 overexpression decreased G1/G0 phase arrest induced by miR-659-3p. *** indicates P < 0.001 in the indicated comparison