Fig. 2
Delivery of PEGFRvIII and FlaB to mouse brain tumor. a Ce6-PEGFRvIII was administrated through different injection methods (intranasal; IN, intravenous; IV, intraperitoneal; IP) and the brain was extracted at 4 h after administration. Image of fluorescence of Ce6-PEGFRvIII in brain tumor was obtained using IVIS spectrum at a wavelength between 640 and 700 nm. b Results are statistically analyzed and an average value of total flux (p/s) is shown (n = 3 for each group). **, p < 0.001. c Tumor is identified by MRI and H&E staining at 2 weeks after EGFRvIII- GL261/Fluc cell implantation. Synthetic Ce6-PEGFRvIII and FlaB were mixed and IN injected (total 20 ul) to C57BL/6 mouse. Signals of Ce6-PEGFRvIII in brains were detected by the IVIS spectrum. To identify FlaB delivery into the brain, paraffin-embedded tissue slides were prepared. FlaB was detected by IF staining using Alexa 488 (green). Each image represents one of three mice