Fig. 5

Biologic effects of HuR down-regulation and rescued by miR-29b-3p inhibitor evaluated in AML cells. a The mRNA expression of HuR after transfection with HuR siRNA and co-transfection with miR-29b-3p inhibitor. b Western blot of HuR expression were detected. The original blots/gels are presented in Supplementary Figure 5. c Grow curve of proliferation based on the OD value at different time points, resceptivly. d Cells were labeled by PI and analyzed using FCM. The percentage of cells in G1/G0, S and G2/M of cell cycle were calculated. e Colonies containing ≥40 cells were counted on day 7~10 using a microscope (×200). f The protein expression of Bcl-2 and Bax were detected by Western blot. The original blots/gels are presented in Supplementary Figure 5. Quantification of Bcl-2 and Bax were normalized to GAPDH and presented in Supplementary Figure 8g-i. g Apoptotic cells were measured by FCM. The total apoptosis rate is equal to the sum of the early apoptosis rate and the late apoptosis rate. h Wright-Giemsa stained invading cells were observed under microscope (×200). The invasion cell numbers were counted under microscope in five HP fields, its statistics are presented in Supplementary Figure 8l. i The OD values (proportional to cell numbers) of migrating cells were measured by MTS assay. (*P<0.05, **P<0.01). The experiments were repeated at least three times