Fig. 4

Biologic effects of inhibition miR-29b-3p evaluated in K562 and U937 cells. a Grow curve of proliferation based on the OD value after miR-29b-3p suppression at different time points, resepctively. b Colonies containing ≥40 cells were counted on day 7~10 using a microscope (×200). c Cells were labeled by PI and analyzed using FCM. The percentage of cells in G1/G0, S and G2/M of cell cycle were calculated. d Apoptotic cells were measured by FCM. The total apoptosis rate is equal to the sum of the early apoptosis rate and the late apoptosis rate. e The protein expression of Bcl-2 and Bax were detected by Western blot. The original blots/gels are presented in Supplementary Figure 4, quantification of Bcl-2 and Bax were normalized to GAPDH and presented in Supplementary Figure 8d-f. f Wright-Giemsa stained invading cells were observed under microscope (×200). The invasion cell numbers were counted under microscope in five HP fields, its statistics are presented in Supplementary Figure 8k. g The OD values (proportional to cell numbers) of migrating cells were measured by MTS assay. (*P<0.05, **P<0.01, vs. NC group). The experiments were repeated at least three times