Fig. 3

OL reversed the EMT and migration of Tu686 and CAL-27 cells induced by TGF-β1. A Morphological changes shown in OL and/or TGF-β1-treated Tu686 (up lane) and CAL-27 (down lane) cells. The cells were treated with PBS, 25 µg/mL OL, 10 ng/mL TGF-β1, and 10 ng/mL TGF-β1 + 25 µg/mL OL for 72 h, respectively. EMT changes were observed under microscope, ×100. B-C Wound-healing scratch assay photos and the statistical result for each group of Tu686 cells under the different treatments (at 24 h). The column chart shows the cell migration of each group of Tu686 cells at 24 h after treatment. *p <0.05 vs. control, and **p <0.05 vs. TGF-β1 group (tests were repeated for 3 times)