Fig. 4

The miR-26a-5p directly regulated the expression of HSDL2 in the Hela and C33A cells. (A) Sequence of HSDL2 3’-UTR binding to the miR-26a-5p was predicted by TargetScan and miRDB database. (B) The free energy score for binding between miR-26a-5p and HSDL2 mRNA was calculated using RNAhybird database. (C) Luciferase activity was analyzed in the Hela cells transfected miR-NC or miR-26a-5p mimic and HSDL2 3’-UTR wild type or mutant. The expression of HSDL2 mRNA and HSDL2 protein were analyzed in the Hela (D and E) and C33A cells (F and G) transfected miR-NC or miR-26a-5p mimic. All data were from three independence experiments (*p < 0.05, t-test)