Fig. 6

Arginine antagonized cell cycle of malignant BMECs. a Detection of LAP3 and HDAC2 expressions in malignant BMECs supplemented with arginine. b Detection of cell proliferation using CCK-8 method. c Arginine supplementation affected the cell cycle distribution of malignant BMECs. Malignant BMECs were treated with 8 mM and 32 mM arginine for 48 h, followed by staining with propidium iodide (PI) for DNA content and cell cycle distribution by flow cytometry. d Western blotting analyzed the effect of arginine supplementation on cyclin A1, cyclin D1, and p27 expressions in malignant BMECs. Malignant BMECs were treated with 8 mM and 32 mM arginine for 48 h, and the expressions of LAP3, HDAC2, cyclin A1, cyclin D1, and p27 were detected by western blotting. Full-length blots are presented in Supplementary Fig. S11 and S15, respectively. The relative level of the target protein was estimated using densitometry and the ratio was calculated relative to the GAPDH control. Bars represent mean values with error bars to represent SD from three independent replicates. Differences between mean values were assessed by two-tailed Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001, compared to control