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Fig. 1 | BMC Cancer

Fig. 1

From: The acidic tumor microenvironment enhances PD-L1 expression via activation of STAT3 in MDA-MB-231 breast cancer cells

Fig. 1

Extracellular acidosis increases PD-L1 expression in MDA-MB-231 cells. A–C The pH of the medium was adjusted by HCl or NaOH, and MDA-MB-231 cells were incubated for 18 h. Protein levels were analyzed by western blotting (A), the culture medium pH was measured using a pH meter (B), and mRNA levels were analyzed by qPCR (C). D–F MDA-MB-231 cells were incubated under acidic conditions for the indicated periods. Protein levels were analyzed by western blotting (D), culture medium pH was measured using a pH meter (E), and mRNA levels were analyzed by qPCR (F)

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BMC Cancer

ISSN: 1471-2407

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