Fig. 1

Amino acid transporter expression analysis showed that SLC6A14 is upregulated in colorectal cancer. A The publicly available TCGA database was used to analyze the mRNA expression levels of SLC6A14, SLC3, and SLC7 family transporters in colorectal cancer. B mRNA expression of SLC6A14, SLC3, and SLC7 family transporters in 28 matched CRC cancer tissues and paired adjacent colon tissues was detected by RT-PCR, and GAPDH was used as the internal control. The P value was calculated by Student’s test. C Representative images of SLC6A14 protein expression in paired normal and colorectal cancer tissues by Western blots. D The relative protein levels of SLC6A14 in the paired normal and colorectal cancer tissues were analyzed, and β-actin was used as the internal control. E The expression of SLC6A14 was evaluated by Western blots in five CRC cell lines (HT29, Caco2, HCT116, SW620 and SW480), and the liver cancer cell line Huh7. The data are expressed as the mean ± SD of three independent experiments. *P < 0.05