Fig. 6

Lyc attenuated PDGFRα activition by binding to it directly. A, B Maestro 2D interactions between PDGFRα and sunitinib (A) or Lyc (B). Residues in green spheres are hydrophobic, blue spheres are polar, red spheres are negatively charged, purple spheres are charged and light yellow spheres are glycine. The purple arrows and their directions represent hydrogen bonds between the ligand and the protein. The green line represents the π-π stacking arrangement seen between the aromatic core. C Kinase activity assays were conducted to validate the inhibition of Lyc on PDGFRα kinase activity. Staurosporine, an effective protein kinase inhibitor, was chosen as positive control. D Biacore analysis results of Lyc and PDGFRα. The black dot represented various concentrations of Lyc, Lyc bound to PDGFRα with a KD of 149.6 uM. F Sensorgram of the interaction between Lyc and PDGFRα. F, G We conducted CETSAs and PDGFRα protein expression was detected by western blotting (F). The line chart showed the relative expression level of PDGFRα (G). Besides, all of the uncropped western blot gels in Supplementary Information 2