Fig. 5

SEM and digital image analysis of mammary gland tissue. SEM analysis of four individual groups [a-control (normal saline, 3 ml/kg, p.o.); b-toxic control (MNU 47 mg/kg, i.v.); c (MNU + Tadalafil; 47 mg/kg i.v. + 2 mg/kg p.o.) and d (MNU + Tadalafil; 47 mg/kg i.v. + 4 mg/kg p.o.)] was performed. Control (a) demonstrated presence of collagenous layers (11), duct (12), nodules (13) and small capillary network (14). MNU (b) administration perceived loss of collagenous covering (11) and formation of tumormicrovessels/small capillary network (14) and nodules (13). Tadalafil treatment restores all the cell organelles close to normal. 3D image reconstruction and software-based analysis dataset of constructs representing score was done by using Image J (NIH) software by thresh holding of stained zones of H&E images followed by pixel vs intensity determination by the 3D interactive surface plot and log-histogram analysis (e,f,g,h and i,j,k,l)