Fig. 2

Validation of dysregulated circRNAs. A Heat map of the six most prominent dysregulated circRNAs between non-cirrhotic and cirrhotic cases. B Agarose gel electrophoresis of PCR products as amplified by divergent and convergent primers of dysregulated circRNAs in cDNA and gDNA with GAPDH used as a reference gene. C and D Expressions of top three prominent up/down-regulated circRNAs in non-cirrhotic HCC samples as validated using qRT-PCR in 20 HCC samples from patients with or without liver cirrhosis. E and F Expressions of top three prominent up/down-regulated circRNAs in non-cirrhotic HCC in 20 HCC samples and paired non-cirrhotic tissues. c, cDNA; g, gDNA