Fig. 4

The identification of circ_0000045-binding RBPs in HNSCC cells. A The predicted association of circ_0000045 with multiple RPBs in HNSCC cells. The potential association of circ_0000045 with RBPs was predicted using the CSCD database (http://gb.whu.edu.cn/CSCD). B A schematic presentation of the circRNA pull-down assay used to purify circ_0000045-associated RBPs in HNSCC cells. C The relative quantitation of proteins eluted with biotin-labeled probes targeting circ_0000045 in SCC-9 cells using a circRNA pull-down, N = 3. D Silver staining of circ_0000045-binding RBPs eluted with biotin-labeled probes in SCC-9 cells during circRNA pull-down. E A mass spectrometric plot, showing the identification of RBPs associated with circ_0000045 in SCC-9 cells. F Functional categorization of circ_0000045-binding RBPs in SCC-9 cells. The enrichment of RBPs in KEGG pathways was conducted using the DAVID system. G Significant enrichment of circ_0000045-binding RBPs from SCC-9 cells in RNA degradation and spliceosome pathways. Investigation of enriched molecular pathways was conducted using the GSEA software. NC-biotin: negative control of biotin-labeled probes targeting circ_0000045; KEGG: Kyoto Encyclopedia of Genes and Genomes; *P < 0.05; **P < 0.01