Fig. 7

Phenotype, persistence and safety of CoG133-CAR T cells. A Flow cytometric analysis showed the phenotype of CAR T and normal T cells from the peripheral blood of Huh7 xenograft mice on day 7. B Statistical analysis showed the proportion of CD3⁺, CD4⁺ and CD8⁺ normal T and CoG133-CAR T cells. Statistics are presented as the means ± SDs. n = 3 per group, n.s. not significant. P < 0.001 vs. the normal T group on CD4/CD8 expression. C CD133 and GPC3 coexpression was detected by flow cytometry. D Statistical analysis showed the CoG133-CAR T cells expression. Statistics are presented as the means ± SDs. n = 3 per group. E We harvested murine organ tissues from four groups of tumor xenograft mice sacrificed 28 days after the second injection of effector cells. The tissues were stained with H&E. The histopathological images of the organ tissues from all groups of tumor-bearing mice showed no differences, and images from the Huh7 group are shown as a representative. Scale bar = 100 µm