Fig. 1

Construction and characterization of two mcDNA vectors and the structure of CoG133-CAR T cells. A Schematic representation of CD133-CAR and GPC3-CAR construction. B The double restriction enzyme digestion for selecting CAR structures. Lanes 1–3 contain three GPC3-CAR-positive bacterial clones, and lanes 4–6 contain three CD133-CAR-positive bacterial clones. M = molecular weight marker. C Schematic diagram showing the generation of the CD133-CAR mcDNA and GPC3-CAR mcDNA. D Electrophoretic analysis to detect mcDNA. After L-arabinose induction, CD133-CAR mcDNA and GPC3-CAR mcDNA were digested into fragments of 5179 bp and 4608 bp, respectively. E Schematic illustration of the CoG133-CAR T cell structure. CD133-CAR T or GPC3-CAR T cells recognized only one tumor cell surface antigen, but CoG133-CAR T cells exerted a destructive effect on tumors containing CD133 and GPC3 antigens by recognizing one of those antigens