Fig. 1
From: Targeting the transcriptional activity of STAT3 by a novel fusion protein
Construction of the KRAB-STAT3 fusion protein. A, Model of KRAB-STAT3 secondary structure. B, Graphic model shows the workflow of the monoclonal cell line screen. C, Immunofluorescence of the expression of STAT3 and Flag in monoclonal Hela cells. K-S: KRAB-STAT3. Bars = 50 um. D, E, Western blot of the expression of KRAB-STAT3 fusion protein in monoclonal HeLa and A375 cells. F, Western blot of Native PAGE of KRAB-STAT3-3xFlag using anti-Flag antibody. G, Co-immunoprecipitation of KRAB-STAT3-3xFlag fusion protein using anti Flag antibody, and endogenous STAT3 was detected using anti-STAT3 antibody at around 86 kDa. H, I, STAT3 and KRAB-STAT3-3xFlag was detected in the cytoplasm, mitochondria, and nucleus using Western blot. Original blots were in Supplementary Fig. 1