Fig. 6
Knocking down LncRNA NDRG1 could inhibit the growth and metastasis of osteosarcoma in vivo. A. 143B cells transfected with a lentiviral vector sh-Lnc-NDRG1 or sh-NC were subcutaneously injected into armpit of nude mice (1 × 107 cells per mice, n = 5 each group). B. The expression of LncRNA NDRG1 in sh-Lnc-NDRG1 group and sh-NC group. C, D. The growth rate and tumor weight obviously decreased in sh-Lnc-NDRG1 group compared with sh-NC group. E. HE and IHC staining of xenograft tumors. The expression of Ki67 were analyzed based on IHC staining. The samples were imaged at 400× magnification. Scale bar = 50 μm. F. Representative image and analysis of luminescence intensity in tail vein-injected mouse models (n = 3 for each group). G, H. Representative image and HE staining of metastatic lung nodules. The HE staining samples were imaged at 40× magnification. Scale bar = 100 μm. I. The number of lung metastatic nodules in sh-NC and sh-NDRG1 group. All data are presented as the means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001. (student’s t test)