Fig. 4

MiR-96-5p suppresses cell proliferation, migration and EMT in vitro. A. The expression level of miR-96-5p was detected in MG63 and 143B cells which transfected with miR-96-5p mimics or inhibitor. B-I. The effects of miR-96-5p mimics and inhibitor on cell proliferation and migration ability were assessed by CCK-8, EdU assays and transwell, wound healing assays in MG63 and 143B. The samples were imaged at 100× magnification. Scale bar = 100 μm. J-K. The expression of EMT related genes in MG63/143B cells transfected with miR-96-5p mimics or inhibitor. All data are presented as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. (student’s t test)