Fig. 6
From: Integrated analysis reveals the dysfunction of signaling pathways in uveal melanoma
The function of the SPP1 signaling pathway in melanocytes. Knockdown efficiency of CD44 (A) and SPP1 (B) in melanocytes. C Cell viability was determined 24 h after CD44 and SPP1 knockdown, followed by a CCK-8 assay. Data were normalized to NC. D Immunofluorescence staining using EdU in CD44- and SPP1-silenced melanocytes. Scale bars: 100 μm. E Data were collected from at least five sections, and cell numbers were averaged. F The migration activity of melanocytes transfected with CD44 siRNA or SPP1 siRNA was measured by a Transwell assay. G Data are shown as the mean ± SD of three independent experiments. **, P < 0.01