Fig. 4

PIK3R3 overexpression in ovarian cancer stem cells. A Correlation analysis between PIK3R3 expression and expression of cancer stem cell-related proteins, such as SOX2, CD44, and ALDH1A1, in ovarian cancer stem cells. The starBase database was used to determine the correlation between PIK3R3 and stem cell-related genes (SOX2, CD44, ALDH1A1). B PIK3R3 expression obtained from the GSE53759 dataset that contains information on monolayer and spheroid-derived cells from ovarian carcinoma IGROV-1 in triplicate. C RT-qPCR analysis of the mRNA levels of PIK3R3 obtained from ovarian cancer (A2780 and SKOV3) and spheroid-derived (A2780-SP and SKOV3-SP) cells (n = 3). Data are represented as the mean ± SD. ***P < 0.001 vs control. D Western blot analysis of protein expression levels of PIK3R3 obtained from ovarian cancer (A2780 and SKOV3) and spheroid-derived (A2780-SP and SKOV3-SP) cells. Lower panels represented densitometric quantification of the western blots. Data are presented as the mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. E The PIK3R3 expression level obtained from the GSE124766 dataset that contains information on HGSOC tumor organoids and tumor tissues compared to FT normal organoids and FT Normal tissues. Data are presented as the mean ± SD from three independent experiments. *P < 0.05