Fig. 2
Ixazomib in combination with STF-083010 arrests cell cycle at G1 phase. Both KMS11 and RPMI-8226 cells were incubated for 16 h in absence of FBS, followed by the treatment strategy described in Fig. 1 for 16 h), (n = 3). A Cells were harvested and DNA content was assessed by PI staining. P values were calculated by one-way analysis of variance (ANOVA). A P value of less than 0.05 was considered statistically significant for all analyses (*p < 0.05, **p < 0.01, ***p < 0.001). B Using western blot, protein levels of cell cycle negative regulators p21CIP1, p27.KIP1 and β-actin as loading control were measured in KMS11 and RPMI-8226 after 16 h of treatment