Fig. 5

Imaging of CDC-mediated apoptosis and necrosis in Raji spheroids treated with RTX. a Z-projected fluorescence confocal microscopy images of Raji spheroids treated with RTX isotypes or isotype control in human plasma, and stained with Annexin V FITC (yellow) and Hoechst 33342 nuclear stain (blue). Profile of the Annexin V FITC fluorescence signal in the different RTX isotype or control-treated Raji spheroids (right panel). b Z-projected fluorescence confocal microscopy images of Raji spheroids treated with RTX isotypes or isotype control in human plasma, and stained with propidium iodide (PI) (pink) and Hoechst (blue). Profile of the PI fluorescence signal in the different RTX isotype or control-treated Raji spheroids (right panel). Data from one representative experiment of three independent experiments is shown. Scale bar =300 μm, magnification 10x. The X-axis in diagram represents the distance from the spheroid border and the Y-axis the pixel intensity