Fig. 1

Generation and characterization of CMs mimicking the BM niche. A) Quantification of different cell populations in expanded BM cultures (n = 6) by flow cytometry (empty dot plots). Purified culture of BM-MSC (column bars) was used as positive control for stroma markers (CD90, CD105 and HLA-DR). B) Graphical scheme of CM’s production (left). In vitro image (10x objective) of cells used for CM’s production at 48 h (right). C) Cytokine Profile Array membranes of CM-NB, CM-BM, CM-BM/NB (left). Quantification of detected cytokines and chemokines represented as mean pixel density of duplicate spots (right). D) Extracellular MIF quantification by ELISA in CM-NB (n = 6), CM-BM (n = 5) and CM-BM/NB (n = 6), being LAN-1 square, SH-SY5Y rhomboid, and IMR5 a circle