Table 1 Antibodies and optimal staining conditions for Western blot analysis
First antibody | Clone no. / Company | Dilution | Secondary antibody |
|---|---|---|---|
Anti-p-AKT Ab | 9271/ Cell Signaling | 1:500 | 4 °C, overnight |
Anti-AKT Ab | 9272/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-p-ERK Ab | 9101/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-ERK Ab | 9102/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-p-mTOR Ab | 2971/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-mTOR Ab | 9272/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-p-S6 Ab | 2215/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-S6 Ab | 2217/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-p-STAT3 Ab | 9131/ Cell Signaling | 1: 1000 | 4 °C, overnight |
Anti-STAT3 Ab | 9139/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-p-GSK-3β Ab | 9336/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-GSK-3β Ab | 9315/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-p-JNK Ab | 9251/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-JNK Ab | 9252/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-p-p38 Ab | 9211/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-p38 Ab | 9212/ Cell Signaling | 1:1000 | 4 °C, overnight |
Anti-PD-L1 Ab | Ab205921/ Abcam | 1:500 | 4 °C, overnight |
Anti-β-actin Ab | Sc-47778/ Santa Cruz | 1:10,000 | 4 °C, overnight |