Fig. 4

Effects of PI3K inhibitor and AKT inhibitor on cell invasion in RAGE KD breast cancer cell lines. Cell invasion was determined with 100 ng/ml rHMGB1 or in a combination with either 150 nM GDC-0941 or 2 μM AT13148 in 1% BSA DMEM for 48 h. Representative images of invaded cells were visualized using an inverted fluorescence microscope, scale bar = 200 μm, and original magnification 40X (a and b). Bar graphs represent percent cell invasion of unstimulated controls (1% BSA DMEM) calculated from the number of invaded cells of 10 images taken from 2 wells per conditions (c and d). *P < 0.05