Fig. 3

Effect of RAGE silencing on rHMGB1-mediated tumor cell invasion. 3-D growth of parental, mock, RAGE-KD cells of MDA-MB-231 P and MDA-MB-231 BM (a and b). Graphs represent the average of six data sets for each condition. The expression levels of RAGE protein in mock and RAGE-KD of MDA-MB-231 P and MDA-MB-231 BM cells was detected by Western blot analysis (c). Representative images of tumor spheroid invasion after 100 ng/ml rHMGB1 treatment of MDA-MB-231 P (d and e) and MDA-MB-231 BM (f and g) are shown. Bars represent mean ± SD of duplicate independent experiments. *P < 0.05; comparing % cell invasion of mock-treated cells with or without 100 ng/ml rHMGB1; ^#P < 0.05; comparing % cell invasion of rHMGB1 treated cells in mock-treated and RAGE-KD cells